Chèvre anti-Souris IgG3 Anticorps (HRP)

N° du produit ABIN1118505

N° du produit (Fournisseur): 610-103-043

Aperçu rapide pour Chèvre anti-Souris IgG3 Anticorps (HRP) (ABIN1118505)

Antigène: IgG3

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Chèvre

N° du produit (Fournisseur): 610-103-043

Fournisseur: Rockland

Fonction: Mouse IgG3 Antibody Peroxidase Conjugated Pre-adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

Attributs du produit: Anti-Mouse IgG2b Peroxidase Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain).

Immunogène:

Immunogen: Mouse IgG3 heavy chain

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Mouse IgG3 peroxidase antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Guo, Kong, Peng, Long, Zhou, Shu: "Transcription factor NF‑YA promotes a malignant phenotype by upregulating fatty acid synthase expression." dans: Molecular medicine reports, Vol. 14, Issue 6, pp. 5007-5014, (2017) (PubMed).

Chen, Ruan, Long, Peng, Zhou, Liu, Zhou, Liu et al.: "Blocking fatty acid synthase inhibits tumor progression of human osteosarcoma by regulating the human epidermal growth factor receptor 2/phosphoinositide 3-kinase/protein kinase B signaling pathway ..." dans: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 2411-2416, (2017) (PubMed).

Détail du antigène

Antigène: IgG3

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.